Comparison of three methods for the detection of Angiostrongylus vasorum in the final host.
Identifieur interne : 000015 ( France/Analysis ); précédent : 000014; suivant : 000016Comparison of three methods for the detection of Angiostrongylus vasorum in the final host.
Auteurs : E. Houpin [France] ; G. Mccarthy [Irlande (pays)] ; M. Ferrand [France] ; T. De Waal [Irlande (pays)] ; E J O'Neill [Irlande (pays)] ; A. Zintl [Irlande (pays)]Source :
- Veterinary parasitology [ 1873-2550 ] ; 2016.
English descriptors
- KwdEn :
- Angiostrongylus (genetics), Angiostrongylus (isolation & purification), Animals, Antigens, Helminth (analysis), Dissection (standards), Enzyme-Linked Immunosorbent Assay (standards), Foxes (parasitology), Molecular Diagnostic Techniques (standards), Parasitology (methods), RNA, Ribosomal, 18S (genetics), Reproducibility of Results, Sensitivity and Specificity, Strongylida Infections (diagnosis).
- MESH :
- chemical , analysis : Antigens, Helminth.
- diagnosis : Strongylida Infections.
- genetics : Angiostrongylus, RNA, Ribosomal, 18S.
- isolation & purification : Angiostrongylus.
- methods : Parasitology.
- parasitology : Foxes.
- standards : Dissection, Enzyme-Linked Immunosorbent Assay, Molecular Diagnostic Techniques.
- Animals, Reproducibility of Results, Sensitivity and Specificity.
Abstract
Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
DOI: 10.1016/j.vetpar.2016.02.023
PubMed: 26995721
Affiliations:
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pubmed:26995721Le document en format XML
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Houpin</name><affiliation wicri:level="1"><nlm:affiliation>Ecole Nationale Vétérinaire de Toulouse (ENVT), France. Electronic address: houpin.emilie@gmail.com.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Ecole Nationale Vétérinaire de Toulouse (ENVT)</wicri:regionArea></affiliation></author><author><name sortKey="Mccarthy, G" sort="Mccarthy, G" uniqKey="Mccarthy G" first="G" last="Mccarthy">G. Mccarthy</name><affiliation wicri:level="1"><nlm:affiliation>School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4</wicri:regionArea><wicri:noRegion>Dublin 4</wicri:noRegion></affiliation></author><author><name sortKey="Ferrand, M" sort="Ferrand, M" uniqKey="Ferrand M" first="M" last="Ferrand">M. 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Zintl</name><affiliation wicri:level="1"><nlm:affiliation>School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: annetta.zintl@ucd.ie.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4</wicri:regionArea><wicri:noRegion>Dublin 4</wicri:noRegion></affiliation></author></analytic><series><title level="j">Veterinary parasitology</title><idno type="eISSN">1873-2550</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Angiostrongylus (genetics)</term><term>Angiostrongylus (isolation & purification)</term><term>Animals</term><term>Antigens, Helminth (analysis)</term><term>Dissection (standards)</term><term>Enzyme-Linked Immunosorbent Assay (standards)</term><term>Foxes (parasitology)</term><term>Molecular Diagnostic Techniques (standards)</term><term>Parasitology (methods)</term><term>RNA, Ribosomal, 18S (genetics)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Strongylida Infections (diagnosis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Strongylida Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Angiostrongylus</term><term>RNA, Ribosomal, 18S</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Angiostrongylus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Parasitology</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Foxes</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Dissection</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.</div></front></TEI><affiliations><list><country><li>France</li><li>Irlande (pays)</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Houpin, E" sort="Houpin, E" uniqKey="Houpin E" first="E" last="Houpin">E. Houpin</name></noRegion><name sortKey="Ferrand, M" sort="Ferrand, M" uniqKey="Ferrand M" first="M" last="Ferrand">M. Ferrand</name></country><country name="Irlande (pays)"><noRegion><name sortKey="Mccarthy, G" sort="Mccarthy, G" uniqKey="Mccarthy G" first="G" last="Mccarthy">G. Mccarthy</name></noRegion><name sortKey="De Waal, T" sort="De Waal, T" uniqKey="De Waal T" first="T" last="De Waal">T. De Waal</name><name sortKey="O Neill, E J" sort="O Neill, E J" uniqKey="O Neill E" first="E J" last="O'Neill">E J O'Neill</name><name sortKey="Zintl, A" sort="Zintl, A" uniqKey="Zintl A" first="A" last="Zintl">A. Zintl</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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